The purpose of the study is to examine the difference of the functional connectivity between the nasal and mouth breathing conditions in healthy subjects using resting-state functional magnetic resonance imaging via seed-based correlation analysis. “Mouth>Nose” contrast had 5 seeds and 23 connecting pairs, however, 6 seeds and 14 pairs in the “Mouth<Nose” contrast. Especially, caudate had the most number of connections of salience networks, supramarginal gyrus, insular cortex, central opercular cortex, supramarginal gyrus, and parietal operculum in “Mouth>Nose” contrast. These indicated that the limbic system regulates the resting-state functional connectivity during the voluntary mouth breathing compared the nasal breathing.
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