Improvements in outcomes with current chemotherapy and surgery treatment approaches for patients with osteosarcoma have plateaued over the past three decades necessitating new treatment paradigms. A novel therapy for osteosarcoma of MR-guided cryotherapy in conjunction with intratumoral injection of an immune adjuvant in the clinically relevant animal model of spontaneously occurring osteosarcoma in client-owned dogs with the intent of priming an adaptive immune response capable of delaying or preventing the occurrence of metastatic disease is demonstrated.
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