MRgFUS ablation of tendons is a promising treatment to disrupt tendons in conditions such as musculotendinous contractures. In this study, DTI and tractography were proposed as tools to assess tendons before and after MRgFUS ablation, providing a quantitative, directional, and visual assessment of tendon tract architecture and integrity. Diffusivity parameters (FA and ADC) changed in accordance to the tendon fascicle integrity. Fiber tractography visually delineated healthy tendon fascicles and confirmed tract disruptions at the same location as registered with b0 and T1-weighted images. This study demonstrates the potential for DTI and tractography as assessment tools for MRgFUS ablation treatments.