White matter injury is common in neonates. The most common punctate white matter lesions (PWML) can disappear along with time and are easily missed diagnosis. Most current clinical scores account for the numbers and the size of PWML, but ignore the lesion location, which is important for the outcome prediction. This study aims to quantitatively assess the distribution of mild PWML in preterm and term neonates, and characterize its dynamic alterations with gestational age. Our study found that mild PWML is mainly located in the posterior of brain, with extensive injury in the preterm extending to the central region.