Sulcal pits is known to represent the first cortical folds emerging in early fetal life. However, little is known about sulcal pits in the fetal brain. Since fetal brain showed dynamic changes of cortical folding during the second half of gestation, it is necessary to investigate spatial and temporal patterns of sulcal pits in the fetal brain. We analyzed spatial distribution and quantified emergence timing of pits using 48 typically developing fetuses. Sulcal pits were uniformly distributed in the fetal brain, and their timing of emergence are regionally different. It demonstrates sulcal pits are important landmarks of human brain development.