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Abstract #4815

 Effect of hypoxia and choline kinase inhibitor on choline metabolism of brain tumour cells using high resolution ¹H NMR

Claire Louise Kelly1, Sofya A Osharovich2, Marie Phelan3, Violaine See3, Edward J Delikatny2, and Harish Poptani1
1Center for Preclinical Imaging, University of Liverpool, Liverpool, United Kingdom, 2Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States, 3Institute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom

High resolution ¹H NMR of cell extracts was used to assess the effects of hypoxia on choline metabolism in four glioblastoma (GBM) cell lines (9L, F98, U87 and U251). In addition, the effects of hypoxia on the efficacy of JAS239, a choline kinase alpha (ChoKα) inhibitor, was also assessed in these cell lines. Hypoxic preconditioning resulted in a decrease in phosphocholine (PC) in 9L, F98 and U251 cells. JAS239 significantly decreased PC/glycerophosphocholine (GPC) ratio in the hypoxic 9L and U251 cells compared to when these cells were grown in normoxic conditions.

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