Abstract #0016

Delayed mapping of 2H-labeled choline using Deuterium Metabolic Imaging (DMI) reveals active choline metabolism in rat glioblastoma.

Henk M. De Feyter¹, Monique A. Thomas¹, Kevan L. Ip¹, Kevin L. Behar², and Robin A. de Graaf^3,4

¹Department of Radiology and Biomedical Imaging, Yale University, New Haven, CT, United States, ²Department of Psychiatry, Yale University, New Haven, CT, United States, ³Department of Radiology and Biomedical Imaging, Yale University, NEW HAVEN, CT, United States, ⁴Department of Biomedical Engineering, Yale University, New Haven, CT, United States

Previously we reported high tumor uptake of exogenous choline in rodent brain tumor models when using Deuterium Metabolic Imaging (DMI) during intravenous infusion of [²H₉]-choline. The small differences in chemical shifts of [²H₉]-labeled choline, phosphocholine, glycerophosphocholine and betaine exclude accurate peak assignment, and therefore it is unclear whether blood-borne choline is metabolized intracellularly. Using different ²H-labeling strategies of choline and high resolution ²H NMR in tumor tissue extracts we identified the choline-containing metabolites observed during intravenous infusion, as well as after 24 hrs.

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