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Abstract #0082

Investigating the microglial metabolome with high resolution 1H NMR

Lydia M. Le Page1, Jayson Ball2, Linda Watkins2, and Myriam Chaumeil1
1Physical Therapy and Rehabilitation Science, Radiology and Biomedical Imaging, UCSF, San Francisco, CA, United States, 2Department of Psychology & Neuroscience & the Center for Neuroscience, University of Colorado, Boulder, CO, United States

Microglia are an essential part of the brain’s immune system. In 2006, Frank et al. described a rapid isolation technique to obtain high-purity quiescent microglia from the rat brain, enabling study of their role in health and disease. Metabolomics via NMR is a robust, data-rich strategy for understanding cell metabolism. Given the central role of microglial metabolic reprogramming in numerous diseases, we investigated whether ultra-high-field 1H NMR could detect key metabolites in freshly isolated adult microglia. We optimized the isolation and extraction protocols and showed that several key microglia metabolites can be reliably quantified using high-resolution 1H NMR at 18.8Tesla.

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