We measured in vivo human brain T2* values using 7T MRI at 0.35 × 0.35 × 0.35 mm3. We simultaneously targeted calcarine sulcus and Heschl’s gyrus. Our results show that gray matter T2* varies up to 15 ms (global range being mostly in between 25-45 ms) from deep to superficial layers. The stria of Gennari shows up as a major reduction of T2* within calcarine sulcus. However, a similar layering is not visible within Heschl’s gyrus. B0 alignment effects seem to be not as strong as the biological tissue composition effects that are observed across the visual and auditory regions.