Abstract #0236

Assessment of sensitivity, resolution, and quantification of Synomag-D labeled dendritic cells with magnetic particle imaging

Julia J Gevaert^1,2, Corby Fink^3,4, Jimmy D Dikeakos³, Gregory A Dekaban^3,4, and Paula J Foster^1,2

¹Department of Medical Biophysics, University of Western Ontario, London, ON, Canada, ²Cellular and Molecular Imaging Group, Robarts Research Institute, London, ON, Canada, ³Department of Microbiology and Immunology, University of Western Ontario, London, ON, Canada, ⁴Biotherapeutics Research Laboratory, Robarts Research Institute, London, ON, Canada

The purpose of this study was to explore magnetic particle imaging (MPI), which directly detects superparamagnetic iron oxide nanoparticles (SPIOs) as a novel technique for tracking dendritic cells (DCs). DCs were labeled using Vivotrax, a commonly used MPI-SPIO, and Synomag-D, a tailored MPI-SPIO, with and without transfection agents. Vivotrax with TAs showed high levels of extracellular iron, overestimating iron content and cellular sensitivities. The use of TAs improved cellular sensitivity of Synomag-D to as few as 6k cells with 1min 2D images. Signal from 250k and 25k cells could be resolved at a distance of 2cm with 3D imaging.

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