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Abstract #0236

Assessment of sensitivity, resolution, and quantification of Synomag-D labeled dendritic cells with magnetic particle imagingĀ 

Julia J Gevaert1,2, Corby Fink3,4, Jimmy D Dikeakos3, Gregory A Dekaban3,4, and Paula J Foster1,2
1Department of Medical Biophysics, University of Western Ontario, London, ON, Canada, 2Cellular and Molecular Imaging Group, Robarts Research Institute, London, ON, Canada, 3Department of Microbiology and Immunology, University of Western Ontario, London, ON, Canada, 4Biotherapeutics Research Laboratory, Robarts Research Institute, London, ON, Canada

The purpose of this study was to explore magnetic particle imaging (MPI), which directly detects superparamagnetic iron oxide nanoparticles (SPIOs) as a novel technique for tracking dendritic cells (DCs). DCs were labeled using Vivotrax, a commonly used MPI-SPIO, and Synomag-D, a tailored MPI-SPIO, with and without transfection agents. Vivotrax with TAs showed high levels of extracellular iron, overestimating iron content and cellular sensitivities. The use of TAs improved cellular sensitivity of Synomag-D to as few as 6k cells with 1min 2D images. Signal from 250k and 25k cells could be resolved at a distance of 2cm with 3D imaging.

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