Mammalian neurons operate at length scales spanning many orders of magnitude; micron-scale-diameter myelinated axons project millimeters across brain regions, forming nanometer scale synapses. Capturing these disparately sized anatomical features requires imaging samples with multiple independent imaging modalities. Directly correlating features across modalities requires that all imaging is performed in the same brain. Here, we imaged the same postmortem mouse brain over five orders of spatial resolution using MRI, synchrotron x-ray tomography (μCT), and electron microscopy. This pipeline provides an unprecedented look across a single brain's multi-scaled organization and a vehicle for studying the brain’s multi-scaled pathologies.