Imaging-guided nose-to-brain drug delivery provide a non-invasive monitoring of drug delivery to brain, which increases effective dose via bypassing Blood-Brain-Barrier(BBB). Here, we investigated imaging of nanomedicine delivery via intranasal-administration using CEST-detectable mucus-penetrating-liposome(with 10%PEG). Liposomes were loaded with Iohexol(Ioh-Lipo) and CEST properties were examined both in-vitro and in-vivo by injecting into mouse nostril. Ioh-Lipo generated CEST contrast of 33.4% at 4.3 ppm in-vitro. which was also observed in nostril, olfactory-bulb and frontal-lobe after intranasal-administration at 3T. We demonstrated the liposomes detectability both in nostril and olfactory-bulb by CEST. The result demonstrates an approach for imaging-guided Nose-to-Brain Intranasal Liposomal Drug Delivery.