Preoperative assessment of histological grades and possible margin of glioma are challenges to neurosurgeons, which may also make a huge difference in therapeutic strategies. In this study, we aim to establish a grade-discrimination criterion via APT and explore the possible metastatic margin of diffuse glioma at 7T. Eleven patients underwent structural imaging and APT CEST imaging. Results of the APT were merged with the T1 image, which showed a promising indication of preoperative histological grades. Meanwhile, with the early exploration of metastatic margin, the APT CEST at 7 Tesla may significantly facilitate the surgical strategy and surgical excision extension.