Lymphovascular invasion (LVI) is associated with increased recurrence and metastatic risk in breast cancer. LVI, with genetic mutation in metabolism of saturated and monounsaturated fatty acids, leads to the deregulation of lipid composition. Currently LVI is estimated using the pre-operative core biopsy, while definitive LVI can only be determined after surgery, precluding patients to receive timely neoadjuvant chemotherapy or hormonal treatment. Double quantum filtered correlation spectroscopy (DQF-COSY) is a method capable of accurate non-invasive lipid composition quantification. We therefore hypothesised there is a difference in lipid composition derived from DQF-COSY between LVI status.