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Abstract #1227

Metabolic Responses of HeLa Cells upon addition of the Photosensitizer Chlorin e4 and Carriers in the Dark by 1H HR-MAS NMR

Martina Vermathen1, Tobias Emmanuel Kämpfer1,2, and Peter Vermathen2
1Department of Chemistry and Biochemistry, University Bern, Bern, Switzerland, 2Departments of BioMedical Research and Radiology, University Bern, Bern, Switzerland

Porphyrinic photosensitizers (PSs) like chlorin e4 (Ce4) are applied with polymer-based carriers for photodynamic therapy of cancer. However, the impact of PSs and their carriers on the metabolic state of cells in the dark-interval before light treatment are not clear. Here, HR-MAS NMR metabolomics was applied to cancer cells treated with Ce4 alone or combined with polyvinylpyrrolidone (PVP) or block-copolymer micelles (BCMs). A Ce4-concentration dependent metabolic response was observed that was clearly attenuated by BCMs and more pronounced by PVP indicating the beneficial role of the carriers. Direct interaction of Ce4 with glycero-phosphocholine was observed suggesting intracellular membrane localization.

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