Porphyrinic photosensitizers (PSs) like chlorin e4 (Ce4) are applied with polymer-based carriers for photodynamic therapy of cancer. However, the impact of PSs and their carriers on the metabolic state of cells in the dark-interval before light treatment are not clear. Here, HR-MAS NMR metabolomics was applied to cancer cells treated with Ce4 alone or combined with polyvinylpyrrolidone (PVP) or block-copolymer micelles (BCMs). A Ce4-concentration dependent metabolic response was observed that was clearly attenuated by BCMs and more pronounced by PVP indicating the beneficial role of the carriers. Direct interaction of Ce4 with glycero-phosphocholine was observed suggesting intracellular membrane localization.
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