Tractography and structural connectome have been widely used in preclinical studies in recent years. However, assessment of the experimental parameters for the final outputs of diffusion MRI is still lack. In this study, we acquired an ex vivo mouse brain high spatial (50 µm isotropic) and angular resolution (384 diffusion encoding directions) diffusion MRI dataset in a preclinical 9.4T system. We analyzed the tractography and connectome under different experimental conditions, including b value, spatial resolution, and angular resolution.
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