Affected by the inherent physical properties, it was challenging to obtain the imaging of heteronuclear at 3T, which usually have weak MR signals. Hence, high performance was required of the RF system to excite and receive MR signals. In this study, a range of imaging tests were performed on the phantom by using the developed specific quadruple-nuclear RF coil system. The results demonstrated the feasibility of synchronized 1H/19F/ 23Na/31P MR imaging at 3T. Furthermore, the imaging approach combined the quadruple-nuclear RF coil and triple tuned local 19F/23Na/31P receive coil was also indicated the improved SNR of corresponding nucleus.