Previously, we demonstrated the feasibility of performing simultaneous MRS localization in two human brain regions using the dual-SPECIAL technique. Based on the SPin ECho, full Intensity Acquired Localized sequence, this approach yields simultaneous acquisition from two regions in combination with Hadamard encoding, reducing the acquisition time by half compared with serial acquisition. Here, we demonstrate that MRS acquisition using dual-Special sequence reveals significant differences in the ratio of tCho/tCr between anterior and posterior regions and higher concentration of several metabolites in regions with higher gray matter fraction.
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