Portal hypertension (PH) is a severe liver disease which could lead to gastroesophageal varices. Noninvasive methods to diagnose PH and to evaluate the risk of variceal bleeding are needed. 4D Flow MRI is an advanced technique which could provide visualization and quantification of blood flow. In the study, we identified the reproducibility of the processing and quantifying procedure of 4D Flow data and discovered the significant hemodynamic differences in portal vein between PH patients and controls using 4D Flow MRI. Additionally, we tested the performance of 4D Flow MRI in diagnosing PH and stratifying the risk of variceal bleeding.