Single voxel MRS pulse sequences of semi-LASER and short-TE STEAM were compared for cerebral glucose quantification via detecting H1-α-glucose peak at 5.23 ppm. Young healthy non-fasted subjects were scanned on a whole-body 7T MRI. Spectra were analyzed on LCModel and in-house MATLAB software. Semi-LASER (32-ms TE) detected the H1-α-glucose peak at posterior cingulate cortex (27-mL volume) with higher SNR (9.1 vs 5.1) than short-TE STEAM (5-ms TE) in shorter scan time (11 vs. 12.5 minutes), respectively, and estimated glucose concentration to be 1.26 mM. More robust water suppression techniques are required to stabilize baselines around the peak.