Low b-value DTI (Low-b DTI) has been recently proposed for investigating the CSF physiology, and was reported to reflect the covariance of velocity distribution of the pseudo-random flow. The purpose of this study was to analyze diffusion properties of Low-b DTI (i.e. diffusion tensor, mean diffusivity, and fractional anisotropy) for normal volunteers. The diffusion properties show statistically high and anisotropic values at some ROIs such as around the foramen of Monro, the aqueduct, the prepontine cistern and the Sylvian fissure. It demonstrates that Low-b DTI can be used for evaluating the pseudo-random flow of CSF.