119 patients with chronic glomerulonephritis (CGN) and 19 healthy controls (HCs) were recruited in this study. Among these patients, 43 patients had kidney biopsy. Patients underwent a native renal T1 map examination within 1 week before kidney biopsy. Clinical information and biopsy pathological scores were collected. Compared to HCs, the T1 values of renal cortex in CGN patients were significantly higher and well correlated with renal function, chronic kidney disease (CKD) stage and renal fibrosis. Therefore, native T1 mapping has demonstrated good diagnostic performance in non-invasive detection of CGN fibrosis.