A MatLab-based inversion recovery sequence simulator/calculator was developed with the purpose of determining and testing optimal parameters for 3D IR acquisitions with the purpose of detecting Multiple Sclerosis lesions in brains. Single inversion FLAIR and dual inversion DIR sequences were studied including a “True-T2” DIR sequence removing the undesired T1 weighting inherent in IR sequences to improve lesion-WM contrast. A T2 preparation phase further helps facilitate T1 suppression. Optimized IR sequences were tested on healthy volunteers and some MS patients, on 1.5 T and 3.0 T MR scanners. Good lesion contrast efficiency with high SNR was found for True-T2 DIR.