Resting-state fMRI in mice became a popular method to investigate brain circuits. However, uncertainty resides over the dynamic effects of anesthetics on brain states and functional connectivity. Using a multimodal approach with optical calcium recordings and RS-fMRI, we assessed brain states and networks while switching anesthetic regimen from 1% isoflurane to a combined 0.2% isoflurane/medetomidine anesthesia. We find that brain states reach a steady state after a short transition time and detected that functional connectivity changes were strongest relative to the 1% isoflurane condition. We conclude that brain states and networks are stable from 30 minutes after switching anesthetic regimen.
How to access this content:
For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.
After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.
After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.
Keywords