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Abstract #2921

Combined RS-fMRI and calcium recordings show stabile brain states in mice after switching anesthetic regimen

Bruno Pradier1,2, Lydia Wachsmuth1, Daniel Segelcke2, Nina Nagelmann1, Esther Pogatzki-Zahn2, and Cornelius Faber1
1Department of Clinical Radiology, University Hospital Münster, Münster, Germany, 2Department of Anesthesiology, University Hospital Münster, Münster, Germany

Resting-state fMRI in mice became a popular method to investigate brain circuits. However, uncertainty resides over the dynamic effects of anesthetics on brain states and functional connectivity. Using a multimodal approach with optical calcium recordings and RS-fMRI, we assessed brain states and networks while switching anesthetic regimen from 1% isoflurane to a combined 0.2% isoflurane/medetomidine anesthesia. We find that brain states reach a steady state after a short transition time and detected that functional connectivity changes were strongest relative to the 1% isoflurane condition. We conclude that brain states and networks are stable from 30 minutes after switching anesthetic regimen.

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