Muscone, a musk odorant component that attracts male mice, binds to olfactory receptors on the olfactory epithelium. Subsequently, activation signals are transferred from the receptors to the olfactory bulb, and then to the olfactory cortex and other brain regions. To detect such odor-evoked activation pathways associated with the perception of odorants and induced behaviors, we employed a method that combines periodic odor stimulation and independent component analysis. In this study, we used medetomidine as the anesthesic. Since medetomidine reportedly influences neural activity in a dose-dependent manner, we investigated the muscone-evoked activations at different levels of medetomidine anesthesia.