Quantitative analysis of T2-weighted (T2W) MR images is hindered by the lack of signal intensity standardization. The tissue T2 values are independent of variations in scanner parameters, but T2 mapping is normally not part of the clinical pathway. Autoref is an automated dual-reference tissue normalization of T2W images developed in our group, aiming to reproduce the prostate T2 values. In this study we measured the prostate T2 value in seven healthy volunteers, and compared them with pseudo-T2 values from Autoref normalized T2W. The Autoref was proven to reproduce prostate T2 values as well as contrast within the prostate zones.