High quality diffusion acquisitions are routinely used to study brain connectivity. In each voxel complex intra-voxel fiber crossings may be captured in Orientation Distribution Functions (ODFs). Direct comparison of ODFs calculated with different methods challenging due to a lack of ground truth. Here, we compare different q-space sampling schemes and ODF-reconstructions on a clinical 3T scanner for a known ground truth of crossing Taxons (textile water filled tubes). This comparison illustrates difficulties separating fibers crossing at less than 45° and estimating relative fiber bundle densities using conventional fiber peak identification. Use of more advanced methods is thus recommended.
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