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Abstract #3414

Detection of alterations in water transport across the cell membrane by filter-exchange spectroscopy

Athanasia Kaika1, Geoffrey J. Topping1, Mathias Schillmaier1, and Franz Schilling1
1Department of Nuclear Medicine, Technical University of Munich, School of Medicine, Klinikum rechts der Isar, Munich, Germany

Filter-Exchange Spectroscopy (FEXSY) is performed using a double-diffusion magnetic resonance pulse sequence, which encodes information dependent on transmembrane water exchange. Permeabilized baker’s yeast cells were examined with a FEXSY sequence and trypan blue staining. Upon permeabilization with isopropanol, TritonX-100 and sonication, the AXR value of yeast cells increased and trypan blue staining verified the membrane permeabilization. In presence of low isopropanol concentration, a progressive increase of the AXR value was observed, which stopped after isopropanol removal. No differences were detected in the trypan blue staining over time, in presence and in absence of isopropanol.

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