IVIM-DKI was used to evaluate clinical utility and diagnostic performance of IVIM-DKI parameters in characterization of prostate cancer(PCa) against benign prostatic hyperplasia(BPH) and healthy tissues. Sixteen patients suffering from PCa and BPH were recruited and underwent IVIM-DKI with routine MR sequences and biopsy. IVIM-DKI signal was modelled using hybrid model with total variation (TV) penalty function which removes any spurious values in parameters adaptively. HY with TV method produced high diagnostic performance (AUC:D=0.94-0.98, f=0.82-0.85 and k=0.85-0.91) than HY method for all tissues and high-quality parameter maps. IVIM-DKI parameters (D,f,k) showed significant differences between tumor and BPH and healthy tissue.