This study apply AFQ, in conjunction with MRtrix3 and ConTrack, to identify 24 major white matter fiber bundles in HCs, patients with XT before and after strabismus surgery. Meanwhile, we evaluate ocular dominance (OD) and stereo acuity of post-operative patients. We found that microstructures (MD) of vision-related fibers changed after surgery. Moreover, these changes were associated with indicators of OD. We infer that microstructural changes of the visual spatially related fiber bundles might contribute to the restoration of stereopsis, and the balanced binocular input may be more conducive to the improvements and restoration of binocular visual function.