Longitudinal simultaneous cortex wide Ca2+ imaging and whole brain functional magnetic resonance imaging in awake mice across the lifespan
Francesca Mandino1, Xilin Shen1, David O'Connor2, Bandhan Mukherjee3, Kristin DeLuca3, Ashley Owens3, An Qu1, John Onofrey1,2,4, Xenophon Papademetris1,2, Stephen M. Strittmatter5, and Evelyn Lake1
1Radiology and Biomedical Imaging, Yale University, New Haven, CT, United States, 2Biomedical Engineering, Yale University, New Haven, CT, United States, 3Neurology and Neuroscience,, Yale University, New Haven, CT, United States, 4Urology, Yale University, New Haven, CT, United States, 5Neurology and Neuroscience, Yale University, New Haven, CT, United States
There is a clear need for imaging protocols in awake animals. Yet, there are only a few approaches in mice, despite their usefulness as translational research subjects. We introduce the first protocol for longitudinal simultaneous fMRI and Ca2+ imaging in awake mice. The same animals are imaged four times (from 4 to 12 months of age) – twice under anesthesia, and twice awake. Our framework includes two acclimation protocols: an initial intensive training followed by a ‘refresher' course. We find a marked improvement in motion between the two awake sessions. Implications for future dual-imaging experiments in awake mice are considered.
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