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Abstract #0447

Time-lapse MRI: in vivo single-cell tracking of patrolling immune cells

Enrica Wilken1, Ina Fredrich1, Asli Havlas1, Felix Freppon1, Max Masthoff1, and Cornelius Faber1
1TRIC, Clinic of Radiology, University Hospital Muenster, Muenster, Germany

Synopsis

We validate that time-lapse MRI is able to resolve and follow single immune cells patrolling the brain vasculature. For that reason, we simulate motion-dependent contrast based on artificial k-space, imitate migrating cells with a rotating phantom system, and track iron-labeled monocytes in vivo with repeated T2* weighted imaging. Furthermore, we show that time-lapse MRI allows to differentiate between different motion patterns non-invasively and with whole-brain coverage, and to study altered motion behavior upon inflammatory stimulus and the onset of immune responses distant from the site of inflammation.

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