Brain iron dyshomeostasis/iron overload has been observed in Alzheimer's disease (AD). Being sensitive to the presence of iron, magnetic resonance imaging (MRI), is often used to study the role of iron in disease. In this study, we determined group differences in iron, R2* and myelin, acquired from the same ex-vivo mid-temporal gyri samples from AD and cognitively normal (CN) subjects. We observed decreased myelin and iron in white matter AD compared to CN. These white matter changes are likely due to a loss of iron-rich oligodendrocytes and demyelination during AD pathogenesis.