MR fingerprinting (MRF) is an advanced quantitative MR technique that allows for efficient acquisition of multiparametric tissue maps. We applied high-resolution 3D MRF to examine T1 and T2 changes in 30 pharmacoresistant focal epilepsy patients with negative MRI, using a voxel-wise group-analysis approach. Forty age-and-gender-matched healthy controls were also included for comparison. Significant T1 increase was detected in the temporal pole, mesial temporal, and superior temporal regions, as well as the orbitofrontal cortex, ipsilateral to the side of the epilepsy. These MRF-detected subtle tissue property changes suggest potential structural damage in the ipsilateral limbic network in MRI-negative pharmacoresistant focal epilepsy.