Meeting Banner
Abstract #0543

1H-14N quadrupolar relaxation of protein backbones: a potential intracellular biomarker of glioma invasion

Hana Lahrech1, Manuel Petit1, Sandra Pierre1, Maxime Leclercq1, Lionel Marc Broche2, and François Berger1
1BrainTech Lab INSERM U1205, Grenoble, France, 2University of Aberdeen, Aberdeen, United Kingdom


In FFC-NMR, R1-dispersion curves of biological systems frequently show quadrupolar peaks (QP) between 1.5 and 3.5 MHz that correspond to the cross relaxation of water 1H and protein 14N. QP of glioma invasion tissues (Glio6 and Glio96 mouse models) and of high glioma proliferation (U87 model) were acquired ex vivo and compared to cell pellets in vitro, showing significant differences. This result suggests that QP signals are intracellular, a result which was confirmed by measurements of cells after trypsin treatment which showed unchanged QP amplitude. This result highlights the potential of the QPs as an intracellular biomarker of glioma invasion.

This abstract and the presentation materials are available to members only; a login is required.

Join Here