5 healthy subjects consistently performed a declined squad training routine for 3 months. Every subject received an MRI scan before undergoing the training and subsequently every month until the end of the training. We observed a decrease in AD, MD and FA over the course of training, with a significant change after 1 month. Muscle volume increased, with changes only apparent at 3 months. Changes in diffusion parameters were predictive of changes in global volume. Our study shows that DTI can be a sensitive tool to study muscle hypertrophy and could be used for early monitoring of training and rehabilitation.
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