Abstract #0834
Assessment of Lipid Biosynthesis and Turnover in Renal Cell Carcinoma Cells and Tissues Using NMR-Resolved Stable Isotope Experiments
Daniel Robert Crooks1, Ye Yang2, Andrew Lane3, Teresa Fan3, Jeffrey Brender4, Murali C Krishna4, and W. Marston Linehan1
1Urologic Oncology Branch, National Cancer Institute, Bethesda, MD, United States, 2National Cancer Institute, Bethesda, MD, United States, 3Center for Environmental Systems Biochemistry, University of Kentucky, Lexington, KY, United States, 4Radiation Biology Branch, National Cancer Institute, Bethesda, MD, United States
Synopsis
NMR-based analyses of lipids can shed insight into the global complement of cellular lipids, and elucidate the fuel sources and pathways contributing to lipid biosynthesis in cells grown in the presence of 13C-labeled fuels. We utilized 1H-13C HSQC NMR analysis of cellular lipids derived from FH- and mtDNA deficient UOK271 cells and observed robust reductive carboxylation of glutamine that resulted in formation and incorporation of 13C-glutamine-derived acetyl groups into lipid chains.
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