Abstract #0834

Assessment of Lipid Biosynthesis and Turnover in Renal Cell Carcinoma Cells and Tissues Using NMR-Resolved Stable Isotope Experiments

Daniel Robert Crooks¹, Ye Yang², Andrew Lane³, Teresa Fan³, Jeffrey Brender⁴, Murali C Krishna⁴, and W. Marston Linehan¹

¹Urologic Oncology Branch, National Cancer Institute, Bethesda, MD, United States, ²National Cancer Institute, Bethesda, MD, United States, ³Center for Environmental Systems Biochemistry, University of Kentucky, Lexington, KY, United States, ⁴Radiation Biology Branch, National Cancer Institute, Bethesda, MD, United States

Synopsis

NMR-based analyses of lipids can shed insight into the global complement of cellular lipids, and elucidate the fuel sources and pathways contributing to lipid biosynthesis in cells grown in the presence of 13C-labeled fuels. We utilized 1H-13C HSQC NMR analysis of cellular lipids derived from FH- and mtDNA deficient UOK271 cells and observed robust reductive carboxylation of glutamine that resulted in formation and incorporation of 13C-glutamine-derived acetyl groups into lipid chains.

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