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Abstract #1071

Hepatic and renal ketogenesis of hyperpolarized (1-13C)butyrate: functional evidence of metabolic zonation

Hikari A. I. Yoshihara1, Rolf Gruetter1, and Verena Hoerr2,3
1LIFMET, EPFL, Lausanne, Switzerland, 2Medical Physics Group, Institute of Diagnostic and Interventional Radiology, Jena University Hospital, Friedrich Schiller University Jena, Jena, Germany, 3Heart Center, Department of Internal Medicine II, University Hospital Bonn, Bonn, Germany


Ketones bodies are an important metabolic fuel, and butyrate is a ketogenic short-chain fatty acid. We report the metabolism of hyperpolarized (1-13C)butyrate in the rat kidney and liver. The main metabolites in the kidney include [1-13C]acetoacetate, [1-13C]butyrylcarnitine and [5-13C]glutamate, and [1-13C]acetylcarnitine, 3-hydroxy[1-13C]butyrate, [5-13C]citrate and [3-13C]acetoacetate are also detectable. Fasting results in significantly lower butyrylcarnitine/[1-13C]acetoacetate and glutamate/[1-13C]acetoacetate ratios. Ketogenesis is observed in the liver and the [1-13C]butyrylcarnitine + [1-13C]acetoacetate signal normalized to butyrate is higher with fasting. The unexpectedly low 3-hydroxy[1-13C]butyrate signals are functional evidence of metabolic zonation, with acetoacetate production and reduction occurring in different cells.

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