CrCEST MRI indirectly measures creatine as it exchanges amine (-NH2) protons with free water in the skeletal muscle. When performed on the calf after plantar flexion exercise, creatine recovery data can be obtained in all four major calf muscles. While 2D CrCEST has been performed in adults before, no studies showing its reproducibility have been published. Here, we show that the essential muscles in plantar flexion movement, gastrocnemius muscles, in the calf are candidates for reproducible CrCEST data.
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