Fast scan downfield MRS for NAD+ quantification in human skeletal muscle in vivo at 7 T
Ravi Prakash Reddy Nanga1, Mark Elliott2, Neil Wilson2, Sophia Swago3, Walter Witschey2, and Ravinder Reddy2
1Perelman School of Medicine at The University of Pennsylvania, Philadelphia, PA, United States, 2Radiology, Center for Advance Metabolic Imaging in Precision Medicine, Perelman School of Medicine at The University of Pennsylvania, Philadelphia, PA, United States, 3Bioengineering, University of Pennsylvania, Philadelphia, PA, United States
1H downfield MRS is an emerging technique for quantification of in vivo NAD+ concentrations in human tissue. Though NAD+ can be detected in the skeletal muscle downfield spectrum (>4.7 ppm) despite its low concentration (<1 mM), quantification of [NAD+] in muscle disorders is further challenged by depleted NAD+ and long scan times. To address this challenge, we developed and optimized a downfield MRS technique for rapid measurement of NAD+ targeting calf skeletal muscle in a large voxel approach. This optimized technique has allowed us rapid measurement of NAD+ and shortened scan time considerably compared to previously published MRS techniques.
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