In this study, we prepared a 15N-enriched analog of NAD+ (N-methyl nicotinamide = MNA+) and demonstrated that it undergoes reduction by sodium dithionate to form MNAH. The 15N chemical shifts of the oxidized and reduced forms differ by 124.2ppm. DNP of MNA+ followed by dissolution and 15N NMR showed a favorable T1 relaxation time of 130s at 1T and 50s at 3T. Deuteration of the methyl protons only increased the T1 of 15N by ~10s. The long T1 of 15N in these NAD+/NADH mimetics and the large chemical shift difference offer the exciting potential for their use as redox sensors.
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