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Abstract #1660

Effects of warm ischemia on myocardial metabolism: a normothermic perfused heart MRS study

Belinda Ding1, Liam A.J. Young1,2, Margaret Huang3,4, Aravinda Page5, Simon Messer5, Mike Murphy3, Justin Perkins6, Elizabeth Tunnicliffe2, Stephen Large5, Christopher T Rodgers1, and Jonathan R Weir-McCall5,7
1Wolfson Brain Imaging Centre, University of Cambridge, Cambridge, United Kingdom, 2Oxford Centre for Magnetic Resonance, University of Oxford, Oxford, United Kingdom, 3MRC Mitochondrial Unit, University of Cambridge, Cambridge, United Kingdom, 4Department of Surgery, University of Cambridge, Cambridge, United Kingdom, 5Royal Papworth Hospital, Cambridge, United Kingdom, 6Royal Veterinary College, London, United Kingdom, 7Department of Radiology, University of Cambridge, Cambridge, United Kingdom

Synopsis

Ischaemia-reperfusion injury is a key pathophysiological process driving cardiac injury in myocardial infarction, heart surgery and transplants. Succinate accumulation is a specific marker of ischaemic injury, and a potential therapeutic target to reduce reperfusion injury.Single-voxel proton spectra were acquired during normal perfusion and during ischaemia in three machine-perfused porcine hearts. During ischaemia, a peak was evident in the expected region of succinate, which was absent during normal perfusion. This was further examined in a phantom model which confirmed the peak location with concentration-signal correlation of R>0.999. MRS detection of succinate holds potential for the detection of ischaemia.

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