Attention deficit hyperactivity disorder (ADHD) is a neurodevelopmental disorder characterized by irregular levels of hyperactivity, impulsivity and inattention. It is commonly linked to dopamine and norepinephrine balance. Several studies suggested connections to γ-amino butyric acid (GABA) and glutamate (Glu) imbalance in the dorsal anterior cingulate cortex (dACC). Here we use 1H-MRS to study GABA and glutamate + glutamine (Glx) levels in the dACC of ADHD patients, before and after pharmacological treatment and compared to healthy adults. We found no metabolic difference between ADHD and control group and no change after amphetamine treatment. We found no metabolic correlation to ADHD scores.