Combining fMRI and fluorescence recordings has become an important tool for neuroimaging. However, hemodynamic changes lead to artifacts in fluorescence recordings, since blood absorbs fluorescence light. We investigated the hemodynamic artifact in functional and pharmacological MRI and fluorescence measurements and present an MRI-based correction method. In both stimulation and lactate injection experiments, hemodynamic artifacts in FRET recordings occurred. These artifacts cannot be removed effectively using a standard (purely optical) correction method. An MRI-based correction presented in this work eliminates the artifact while the expected fluorescence signal changes persist.
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