Meeting Banner
Abstract #1992

Highly sensitive “off/on” EPR probes to monitor enzymatic activity by OMRI

Simonetta Geninatti Crich1, Sabrina Geninatti Elkhanoufi2, Diego Geninatti Alberti2, Eric Thiaudiere3, Rachele Stefania1, Elodie Parzy3, Philippe Mellet3, Philippe Massot3, and Silvio Aime1
1University of Torino, Torino, Italy, 2University of torino, Torino, Italy, 3University of Bordeaux-CNRS, Bordeaux, France


New stable organic radicals probes have been proposed in this study to perform Molecular Imaging by monitoring by Overhauser Magnetic Resonance Imaging (OMRI) the enzymatic activity. The radicals used are Tempo-containing esters forming stable micelles that are practically EPR silent. The hydrolysis of the ester bond catalyzed by Carboxyl esterases generates a narrow and intense EPR signal as a consequence of the release of the nitroxide radical from the micelle. Thus we have prepared a off/on probe responsive to the carboxylesterase activity that can be detected quantitatively in the OMR image.

This abstract and the presentation materials are available to members only; a login is required.

Join Here