We have developed a novel 3T 1H-MRS sequence to detect acetylcarnitine. Acetylcarnitine has been investigated by 1H-MRS in the muscle and is thought to play an important role in maintaining metabolic flexibility and insulin sensitivity. As these are hallmarks of metabolic disease, techniques to study acetylcarnitine levels non-invasively are of great interest. The advantage of our approach is the absence of a subtraction scheme and high signal intensity, while both water and lipid resonances are sufficiently suppressed to uncover the acetylcarnitine resonance. This makes our sequence suitable also for detection in tissues susceptible to respiratory motion, such as the liver.