Pelvic floor disorders are prevalent diseases and only 2D dynamic observations of straining exercises at excretion are available in clinics. The understanding of three-dimensional pelvic organs mechanical defects is not yet achievable. We proposed a complete methodology for the 3D representation of the bladder combined with 3D representation of the location of the highest strain areas on the organ surface. We assessed the potential of our method on eight control subjects for the reconstruction of bladder during intense straining from forced breathing exercises. The proposed methodology provided, for the first time, a proper 3D+t spatial tracking of bladder non-reversible deformations.