Lower body negative pressure (LBNP) is a non-invasive physiological method known to decrease cerebral blood flow. This technique was used to study the brain’s response to altered cerebrovascular hemodynamics and showed that, on average, brain stiffness decreases by 2.28% in response to ten minutes of LBNP application and decreased cerebral blood flow. By decreasing and increasing cerebral blood flow by turning the LBNP on and off repeatedly in 10-minute blocks, brain stiffness was shown to increase by 3.02% on average from the initial application of LBNP to the final application of LBNP.
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