13C Magnetic Resonance Spectroscopy (MRS) in the liver provides the only non-invasive method of studying metabolites in vivo, with standard protocols using multiple averages during a free breathing period. Using a respiratory belt to monitor the breathing cycle and in-house scripts, spectra were binned into inhale and exhale groups categorised as spectra within 2 standard deviations of the average minima/maxima. A systematic reduction in B0 during exhale compared with inhale but minimal impact on average B1 values during an inhale versus exhale. However, no significant changes in Phase angle, area under the curve or FWHM between the data sets.
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