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Abstract #2419

Selective galactose culture condition reveals distinct metabolic signatures in complex I and V deficient human skin fibroblasts by 1H HR-MAS NMR

Christoph Meyer1,2,3, Damian Hertig1,2, Christian Urzi1,2,3, Janine Arnold2, Jean-Marc Nuoffer2,4, and Peter Vermathen1
1Magnetic Resonance Methodology, Institute of Diagnostic and Interventional Neuroradiology, University Bern, Bern, Switzerland, 2Institute of Clinical Chemistry, University Hospital Bern, Bern, Switzerland, 3Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland, 4Department of Pediatric Endocrinology, Diabetology and Metabolism, University Children’s Hospital of Bern, Bern, Switzerland


Mitochondrial respiratory chain defects present as highly heterogeneous disorders which cannot be unambiguously diagnosed using standard laboratory methods. In this study we observed the biochemical consequences of complex I and complex V deficient human skin fibroblasts when cultivated under galactose stress condition compared to glucose based cell culture condition. We investigated extracellular flux using Seahorse XFe96 cell analyzer and assessed the metabolome fingerprints using High Resolution Magic Angle Spinning NMR. The selective culture method reveals CI and CV defect-specific changes in metabolites associated with the TCA cycle, malate aspartate shuttle and choline metabolism.

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